MINTON MMP 3017 DRIVER

The annealing temperature of the oligonucleotides with the corresponding seats on the DNA should be approximately the same for each oligonucleotide. The invention also provides a pharmaceutical composition of the invention for the prevention or treatment of infection by bacteria of the genus Listeria or by an associated microorganism. By representative fragment is meant in particular a nucleic acid sequence encoding a biologically active fragment of a polypeptide as defined below. One such method of preparation is also an object of the invention. In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or a fragment thereof involved in the adaptation to atypical conditions. Antibodies according to the present invention can also be used to detect expression of a gene of Listeria monocytogenes imiocua or 4b or related microorganisms. Such recording media are also subject of the invention.

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II must be stably maintained in the host cell and may optionally possess particular signals specifying the secretion of the translated protein.

CA2424952A1 – Listeria inocua, genome and applications – Google Patents

This technique leads to the expression of the vaccine protein in situ and to a 25 immune response of cellular type CTL and humoral antibody. In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or a fragment thereof involved in machinery 10 cell.

The single-stranded DNA templates or RNA thus obtained are deposited on the microarray, under conditions which allow their specific hybridization to the immobilized primers. Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a specific polypeptide of Listeria innocua or monocytogerzes 4b or a fragment thereof.

Preferably, the mnp relates to a nucleotide sequence according to the invention, characterized in that it encodes a polypeptide of Listeria innocua or monocytogenes 4b or a fragment thereof involved in regulatory functions.

The vectors according to the invention are, for example plasmid or viral vectors. Such a method according to the invention-including the identification of specific sequences of L. Such a vaccine will advantageously comprise, besides the recombinant vector, a saline solution, for example a sodium chloride solution.

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Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it encodes a polypeptide of Listeria innocua or monocytogenes 4b or a fragment thereof involved in energy metabolism. Approximately 10 ug of DNA was broken by nebulization 1 minute under a pressure of 1 bar Buchrieser, C. The vector should m,p include a promoter, signals for initiation and termination of translation, as well as appropriate regions of regulation of transcription.

In an equally preferred embodiment, the method according to the invention is characterized in that the specific nucleotide sequences of L.

CAA1 – Listeria inocua, genome and applications – Google Patents

One can, for example, refer to the technique described in US Patent No. I, in fact overlap respectively the genome of Listeria innocua and Listeria monocytogenes 4b. I1 should be understood that the invention does not include polypeptides as 5 natural, that is to say they are not in their natural environment. All of these observations indicates that the genes identified as variables between L. These chips or filters differ mainly by their size, material of the carrier and optionally the number of DNA sequences attached thereto.

Nucleotide sequences encoding 30117 polypeptides described above are also an object of the invention. Simplistically, one fixed representative peptides of different proteins of an organism on a support. By representative fragment is meant in particular a nucleic 0317 sequence encoding a biologically active fragment of a polypeptide as defined below.

The hybrid molecules synthesis methods include the methods used in genetic engineering to construct hybrid nucleotide sequences jmp for the polypeptide sequences sought. According to another embodiment mminton the vaccine composition according to the invention, the nucleotide sequence, preferably a DNA, is complexed with DEAE-dextran, with nuclear proteins or with lipids encapsulated in miton or introduced in the form of a gel facilitating its transfection into the cells.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide Listenia innocua or monocytogenes 4b or a fragment thereof in sensitivity medicines and the like.

To set said maximum homology window, one can use the same algorithms as for the nucleic acid sequences.

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The present invention also relates to the genomic sequence and nucleotide sequences encoding polypeptides of Listeria inrzocua, such as cellular envelope polypeptides, secreted or specific, or involved in the metabolism and the replication process, as well as vectors including the said sequences and cells or animals transformed with these vectors.

Thus, in order to develop and produce its properties, any organization needs to interactions between different metabolic pathways. It is thus possible to replace leucine by valine or isoleucine, aspartic acid by glutamine acid, glutamine by asparagine, arginine by lysine, ete. These different elements are chosen and optimized by the skilled person depending on the host cell used. October 2, under the number I as well as all genes and regulatory sequences contained in said non-coding genome.

Antibodies according to the present invention can also be used to detect expression of a gene of Listeria monocytogenes imiocua or 4b or related microorganisms. Then, it puts the said support into contact with labeled proteins, and after an optional rinsing step, detecting the interaction between said labeled proteins and peptides attached to the protein chip. Les vecteurs selon l’invention sont par exemple des vecteurs d’origine plasmidique ou virale.

It also enables quick identification of differences between the genomes of strains used in multiple industrial applications.

A protein chip described above can be used for the detection of gene products, to establish an expression profile of said genes, in addition to a DNA chip according to the invention. The preparation of these filters or chips is to synthesize oligonucleotides corresponding to the 5 ‘and 3’ of the genes or more internal fragments to amplify fragments of an appropriate size, for example between approximately and bases. The numbering of nucleotide sequences ORFs which will be used subsequently in the present description corresponds to the numbering of the amino acid sequences of proteins encoded by said ORFs.